T7 RNA Polymerase is a highly specific RNA polymerase derived from bacteriophage T7. It efficiently catalyzes the transcription of DNA templates into RNA in vitro by recognizing the T7 promoter sequence. It is widely used in various research and industrial applications related to in vitro transcription.

This enzyme is encoded by T7 phage DNA. It synthesizes RNA complementary to the single-stranded DNA downstream of the T7 promoter sequence, using double-stranded DNA containing the T7 promoter sequence as a template and NTPs as substrates. The enzyme exhibits high specificity for the T7 promoter sequence and does not recognize promoters from other biological sources.